3-nitroY | Nitrotyrosine

1 mg/ml of Protein G purified IgG2A in PBS pH 7,4, 0,09 % sodium azide, 50 % glycerol,The antibody recognizes 3-nitrotyrosine moieties. No detectable crossreactivitywith non-nitrated tyrosine. Not species specific. 0.7μg/ml is sufficient for detection of 5 μg SIN-1 treated BSA by Western Blot..ECL.Antibody works paraffin-embedded sections.

Nitrotyrosine is a marker of nitric oxide dependent oxidative stress. It is a product of tyrosine nitration mediated by active nitrogen. Protein tyrosine nitration results in post-translational modification, a component of nitric oxide signaling.