2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, chloroplastic (ISPF), Recombinant Protein
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2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, chloroplastic (ISPF), Recombinant Protein

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Cat: RP01139
Size: 0.02 mg (E-Coli)/ 0.1 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 1 mg (E-Coli)/ 0.1 mg (Baculovirus)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, chloroplastic (ISPF)
Product Gene Name
ISPF recombinant protein
Product Synonym Gene Name
ISPF
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
AASSAVDVNE SVTSEKPTKT LPFRIGHGFD LHRLEPGYPL IIGGIVIPHD RGCEAHSDGD VLLHCVVDAI LGALGLPDIG QIFPDSDPKW KGAASSVFIK EAVRLMDEAG YEIGNLDATL ILQRPKISPH KETIRSNLSK LLGADPSVVN LKAKTHEKVD SLGENRSIAA HTVILLMKK
Sequence Positions
53-231, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
23,975 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

NCBI/Uniprot Data

NCBI Accession #
NP_001319313.1
NCBI GI #
1063678255
NCBI GenBank Nucleotide #
NM_001334142.1
NCBI GeneID
842700
NCBI Official Full Name
isoprenoid F
NCBI Official Symbol
ISPF
NCBI Official Synonym Symbols
2C-METHYL-D-ERYTHRITOL 2; 4-CYCLODIPHOSPHATE SYNTHASE; isoprenoid F; MECPS; T12P18.1; T12P18_1
NCBI Protein Information
isoprenoid F
NCBI Summary
Encodes a protein with 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity. The protein's activity was confirmed by heterologous expression of phenotypic complementation of the E. coli ispF mutant. Plants defective in this gene display an albino lethal phenotype.Homolog of E. coli IspF
UniProt Gene Name
ISPF
UniProt Synonym Gene Names
MCS; MDS; MECDP-synthase; MECPS; MECS
UniProt Protein Name
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, chloroplastic
UniProt Primary Accession #
Q9CAK8
UniProt Secondary Accession #
Q8LE87; Q9GDE3; Q9SH48
UniProt Related Accession #
Q9CAK8
UniProt Comments
Enzyme of the plastid non-mevalonate pathway for isoprenoid biosynthesis that converts 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate into 2C-methyl-D-erythritol 2,4-cyclodiphosphate and CMP. Also converts 4-diphosphocytidyl-2C-methyl-D-erythritol into 2C-methyl-D-erythritol 3,4-cyclophosphate and CMP. Is essential for chloroplast development.

For research use only, not for clinical use.