14-3-3-like protein GF14 lambda (GRF6), Recombinant Protein
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14-3-3-like protein GF14 lambda (GRF6), Recombinant Protein

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Cat: RP00935
Size: 0.02 mg (E-Coli)/ 0.1 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 1 mg (E-Coli)/ 0.1 mg (Baculovirus)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana 14-3-3-like protein GF14 lambda (GRF6)
Product Gene Name
GRF6 recombinant protein
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MAATLGRDQY VYMAKLAEQA ERYEEMVQFM EQLVTGATPA EELTVEERNL LSVAYKNVIG SLRAAWRIVS SIEQKEESRK NDEHVSLVKD YRSKVESELS SVCSGILKLL DSHLIPSAGA SESKVFYLKM KGDYHRYMAE FKSGDERKTA AEDTMLAYKA AQDIAAADMA PTHPIRLGLA LNFSVFYYEI LNSSDKACNM AKQAFEEAIA ELDTLGEESY KDSTLIMQLL RDNLTLWTSD MQEQMDEA
Sequence Positions
1-248, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
30,991 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
14-3-3-like protein

NCBI/Uniprot Data

NCBI Accession #
NP_001190276.1
NCBI GI #
334187583
NCBI GenBank Nucleotide #
NM_001203347.1
NCBI GeneID
830909
NCBI Official Full Name
G-box regulating factor 6
NCBI Official Symbol
GRF6
NCBI Official Synonym Symbols
14-3-3 PROTEIN G-BOX FACTOR14 LAMBDA; 14-3-3lambda; 14-3-3LAMBDA; AFT1; F12B17.200; F12B17_200; G-box regulating factor 6; GF14 LAMBDA
NCBI Protein Information
G-box regulating factor 6
NCBI Summary
Encodes a member of the 14-3-3 gene family that is a lambda isoform (14-3-3lambda). Interacts with APX3 (ascorbate peroxidase) and AKR2, suggesting a role in mediating oxidative metabolism in stress response. This protein was shown to colocalize and interact with SERK1 by which it is phosphorylated. This protein is also reported to interact with the phosphorylated form of the BZR1 transcription factor involved in brassinosteroid signaling and may affect the nucleocytoplasmic shuttling of BZR1. Interacts with JAZ10.4 which lacks the Jas motif.
UniProt Gene Name
GRF6
UniProt Synonym Gene Names
AFT1; RCI2
UniProt Protein Name
14-3-3-like protein GF14 lambda
UniProt Synonym Protein Names
14-3-3-like protein AFT1; 14-3-3-like protein RCI2; General regulatory factor 6
UniProt Primary Accession #
P48349
UniProt Secondary Accession #
P42647; Q9LX97
UniProt Related Accession #
P48349
UniProt Comments
Is associated with a DNA binding complex that binds to the G box, a well-characterized cis-acting DNA regulatory element found in plant genes . Specific negative regulator of slow-vacuolar (SV) ion channel. Mediates F-actin dynamics possibly through inhibiting ADF1 phosphorylation (PubMed:26345162). Negative regulator of freezing tolerance that modulates cold-responsive C-repeat-binding factors (CBF) DREB1A AND DREB1B proteins stability by facilitating their ubiquitin-mediated degradation when activated by CRPK1-mediated phosophorylation in freezing conditions (PubMed:28344081).

For research use only, not for clinical use.