1 | PEB (4x) | protein extraction buffer

It is recommended to include a protease inhibitor (not supplied with this buffer) from a freshly made stock while preparing the ready-to-use 1x PSB. PEB has been optimized for quantitative small-scale preparation of whole protein extracts from plant/algal tissue. Extraction using the procedure described below will result in maximum yield of proteins and diminish protein degradation and aggregation. Extracts may be quantified using detergent (LDS) compatible methods and have been shown to give highly reproducible and quantitative results in subsequent SDS PAGE gel electrophoresis, Western Blotting, and immunoprecipitation. PEB has been tested on a wide range of species and tissues from higher plants, mosses, lichens, algae, diatoms, dinoflagellates, and cyanobacteria.

PEB is an extraction buffer used to destroy and dissolve total proteins in plant tissues and algal cells. Compared to other cell destruction methods, anionic detergent LDS used in conjunction with a recommended procedure (a combination of ultrasound and freeze/thaw cycles) has been shown to increase the amount of dissolved and undegraded proteins (see References). For 1-2 samples, the recommended procedure has an estimated hands-on time of 20-30 minutes. The expected yield will be 1.5-6µg/µl total protein (recovered from standard procedures), depending on the starting material, such as its biological stage, the homogenization method used (heated head vs ultrasound).