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PI3 Kinase p85β Antibody
SPA-08896
| Size | Price |
| 100 µL | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | PI3K |
| Gene Abbr. | PIK3R2 |
| Gene ID | 5296 |
| Full Name | phosphoinositide-3-kinase regulatory subunit 2 |
| Alias | MPPH, MPPH1, P85B, p85, p85-BETA |
| Introduction | Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival. PTEN reverses this process, and research studies have shown that the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN. PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β. In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins. |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | 572001 |
| Isotype | IgG2B |
| Immunogen | E. coli-derived recombinant human PI 3‑Kinase p85 beta. Lys516-Gly642. O00459. |
| Usage | |
|---|---|
| Application | WB, IF |
| Dilutions | Western Blot (2 µg/mL); Immunofluorescence (8-25 µg/mL) |
| MW(KDa) | 85 |
| Reactivity | Human |
| Specificity | Detects human PI 3‑Kinase p85 beta in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) PI 3-Kinase p85 beta (aa 325-428), rhPI 3-Kinase p85 alpha, rhPI 3-Kinase p87, rhPIK3R4, or rhPIK3R5 is observed. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.