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PI3 Kinase p101 Antibody
SPA-08854
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | PI3K |
| Gene Abbr. | PIK3CA |
| Gene ID | 5290 |
| Full Name | phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha |
| Alias | CLAPO, CLOVE, CWS5, MCAP, MCM |
| Introduction | Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival. PTEN reverses this process, and research studies have shown that the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN. PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β. In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins.The p101 regulatory subunit binds to Gβγ, released from heterotrimeric G proteins, and recruits bound p110γ catalytic subunit to the plasma membrane, which is required for GPCR-induced PI3Kγ activity. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | D32A5 |
| Immunogen | Synthetic peptide corresponding to residues surrounding Gly508 of human PI3 Kinase p101 protein. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly508 of human PI3 Kinase p101 protein. |
| Usage | |
|---|---|
| Application | WB |
| Dilutions | Western Blot (1:1000) |
| MW(KDa) | 101 |
| Reactivity | Human, Mouse |
| Sensitivity | Endogenous |
| Specificity | It recognizes endogenous levels of total PI3 Kinase p101 protein. This antibody also detects a strong background band of unknown origin between 50-60 kDa. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.