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Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody
SPA-11903
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Zap-70 |
| Gene Abbr. | ZAP70 |
| Gene ID | 7535 |
| Full Name | zeta chain of T cell receptor associated protein kinase 70 |
| Alias | ADMIO2, IMD48, SRK, STCD, STD |
| Introduction | The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement. Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck. Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | 65E4 |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Tyr319 of human Zap-70. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr319 of human Zap-70. |
| Usage | |
|---|---|
| Application | WB, IF, FC |
| Dilutions | Western Blot (1:1000) |
| MW(KDa) | 70 (Zap-70), 72 (Syk) |
| Reactivity | Human, Mouse |
| Sensitivity | Endogenous |
| Specificity | It detects endogenous levels of Zap-70 only when phosphorylated at Tyr319. It cross-reacts with endogenous levels of Syk when phosphorylated at Tyr352. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.