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Phospho-Tuberin/TSC2 (Ser1387) Antibody
SPA-11482
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | TSC2 |
| Gene Abbr. | TSC2 |
| Gene ID | 7249 |
| Full Name | TSC complex subunit 2 |
| Alias | LAM, PPP1R160, TSC4 |
| Introduction | Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development. Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors. Tuberin is directly phosphorylated at Thr1462 by Akt/PKB. Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity. In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation), and eventual inhibition of translation.Phosphorylation of tuberin by AMPK at Ser1387 is necessary for cell size control in response to energy deprivation and protects from apoptosis. Furthermore, phosphorylation at Ser1387 primes phosphorylation by GSK3 of upstream sites (Ser1383, Ser1379 and Ser1375), integrating Wnt signaling. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | D2R3A |
| Immunogen | Synthetic peptide corresponding to residues surrounding Ser1387 of human tuberin protein. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser1387 of human tuberin protein. |
| Usage | |
|---|---|
| Application | WB, IP |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:50) |
| MW(KDa) | 200 |
| Reactivity | Human, Mouse, Rat |
| Sensitivity | Endogenous |
| Specificity | It detects endogenous levels of tuberin protein only when phosphorylated at Ser1387. This antibody may also cross-reacts with an unidentified 140 kD protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.