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Phospho-Topoisomerase IIα (Ser1469) Antibody
SPA-11176
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Topoisomerase |
| Gene Abbr. | TOP2A |
| Gene ID | 7153 |
| Full Name | DNA topoisomerase II alpha |
| Alias | TOP2, TP2A |
| Introduction | DNA topoisomerase is an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | D4F5 |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Thr356 of human p90RSK3 protein. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr356 of human p90RSK3 protein. |
| Usage | |
|---|---|
| Application | WB, IP, IF, IHC |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:100) |
| MW(KDa) | 90 |
| Reactivity | Human, Monkey |
| Sensitivity | Endogenous |
| Specificity | It recognizes endogenous levels of p90RSK1 protein when phosphorylated at Thr359. This antibody also detects p90RSK2 phosphorylated at Thr365 and p90RSK3 phosphorylated at Thr356. Phosphorylation of p90RSK isoforms at a proximal serine residue (Ser363 of p90RSK1, Ser369 of p90RSK2, and Ser360 of p90RSK3) does not affect the ability of this antibody to detect the phospho-Thr residue. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.