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Phospho-Smad5 (Ser463/465) Antibody
SPA-10108
| Size | Price |
| 100 µL | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | SMAD |
| Gene Abbr. | SMAD5 |
| Gene ID | 4090 |
| Full Name | SMAD family member 5 |
| Alias | DWFC, JV5-1, MADH5 |
| Introduction | Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Briefly, activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved SSXS motif at the carboxy-terminus of the proteins. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad, Smad4, and together the complex moves to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | SY09-03 |
| Isotype | IgG |
| Immunogen | Synthetic phospho-peptide surrounding S463+S465 of Smad5. |
| Usage | |
|---|---|
| Application | WB, IF, IHC |
| Dilutions | Western Blot (1:1000-1:2000); Immunofluorescence (1:50-1:200); Immunohistochemistry (1:50-1:200) |
| MW(KDa) | 60 |
| Reactivity | Human, Mouse, Rat |
| Storage & Handling | |
|---|---|
| Storage Buffer | TBS (pH 7.4), 1% BSA, 40% Glycerol. |
| Preservative | 0.05% Sodium Azide |
| Storage Temp. | Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.