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Phospho-S6 Ribosomal Protein/RPS6 (Ser235/236) Antibody
SPA-09824
| Size | Price |
| 100 µg | Online Inquiry |
| 25 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | S6 Ribosomal Protein |
| Gene Abbr. | RPS6 |
| Gene ID | 6194 |
| Full Name | ribosomal protein S6 |
| Alias | S6 |
| Introduction | One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation. Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions. These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation. Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | Phosphopeptide containing human, mouse and rat Ribosomal Protein S6 S235/S236 sites. |
| Usage | |
|---|---|
| Application | WB, IHC |
| Dilutions | Western Blot (0.5 µg/mL); Immunohistochemistry (5-15 µg/mL) |
| MW(KDa) | 32 |
| Reactivity | Human, Mouse, Rat |
| Specificity | Detects human, mouse, and rat ribosomal protein S6 when dually phosphorylated at S235 and S236. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.