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Phospho-S6 Ribosomal Protein/RPS6 (Ser235) Antibody
SPA-09818
| Size | Price |
| 0.1 mL | Online Inquiry |
| 0.025 mL | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | S6 Ribosomal Protein |
| Gene Abbr. | RPS6 |
| Gene ID | 6194 |
| Full Name | ribosomal protein S6 |
| Alias | S6 |
| Introduction | One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation. Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions. These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation. Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | MC27 |
| Isotype | IgG |
| Immunogen | Synthetic peptide containing the sequence RRL[pS]SL in which pS corresponds to phospho-serine at residue 235 of human ribosomal protein S6. The immunizing sequence is identical in mouse, rat, chicken and Xenopus. |
| Usage | |
|---|---|
| Application | WB |
| Dilutions | Western Blot (1:500-1:2000) |
| MW(KDa) | 32 |
| Reactivity | Human, Mouse, Rat |
| Specificity | Recognizes ribosomal protein S6 only when phosphorylated on Ser235. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Tissue culture supernatant. |
| Preservative | 0.05% Sodium Azide |
| Storage Temp. | Aliquot and store at -20 °C or -80 °C. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.