Phospho-PRAS40 (Thr246) Sandwich ELISA Kit - CD BioSciences

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Phospho-PRAS40 (Thr246) Sandwich ELISA Kit

Phospho-PRAS40 (Thr246) Sandwich ELISA Kit

SPK-00943

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1 X 96T Online Inquiry
5 X 96T Online Inquiry
10 X 96T Online Inquiry
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Target Information
Target Name PRAS40
Gene Abbr. AKT1S1
Gene ID 84335
Full Name AKT1 substrate 1
Alias Lobe, PRAS40
Introduction Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt. One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 proteins. PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex. Inhibition of mTOR signaling stimulates PRAS40 binding to mTOR, which in turn inhibits mTOR activity. PRAS40 interacts with raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1. mTORC1 in turn phosphorylates PRAS40 at Ser183.
Product Details
Measurement Sandwich
Usage
Application ELISA
Reactivity
Sensitivity This kit detects endogenous levels of PRAS40 protein when phosphorylated at Thr246.

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