Online Inquiry
Phospho-PP1α (Thr320) Antibody
SPA-09194
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | PP1α |
| Gene Abbr. | PPP1CA |
| Gene ID | 5499 |
| Full Name | protein phosphatase 1 catalytic subunit alpha |
| Alias | PP-1A, PP1A, PP1alpha, PPP1A |
| Introduction | Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is highly conserved in eukaryotic cells. Four isoforms of PP1 have been characterized: PP1α, PP1δ, PP1γ1 and PP1γ2. Involvement in cell cycle regulation is one of the biological functions of PP1. It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis. A cell cycle-dependent phosphorylation at Thr320 of PP1α by cdc2 kinase inhibits PP1α activity. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Thr320 of human PP1α. |
| Source/Purification | Antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr320 of human PP1α. Antibodies are purified by protein A and peptide affinity chromatography. |
| Usage | |
|---|---|
| Application | WB, IHC |
| Dilutions | Western Blot (1:1000) |
| MW(KDa) | 38 |
| Reactivity | Human, Mouse, Rat, Monkey |
| Sensitivity | Endogenous |
| Specificity | It detects PP1α only when phosphorylated at Thr320. This antibody may cross-react with the phosphorylated δ or γ isoforms of PP1. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol. |
| Preservative | No Preservative |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.