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Phospho-PLCγ1 (Ser1248) Antibody
SPA-09137
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | PLCγ1 |
| Gene Abbr. | PLCG1 |
| Gene ID | 5335 |
| Full Name | phospholipase C gamma 1 |
| Alias | NCKAP3, PLC-II, PLC1, PLC148, PLCgamma1 |
| Introduction | Phosphoinositide-specific phospholipase C. Phosphorylation at tyrosine residues by both receptor and non-receptor tyrosine kinases results in robust activation of PLCγ1 activity, leading to generation of second messengers. In response to agonists, PLCγ1 is phosphorylated on Tyr783, Tyr711, and Tyr1253 (Tyr753, Tyr759, and Tyr1217 in PLCγ2) resulting in robust PI-4,5-P2 hydrolysis. Interestingly recent evidence suggests a role for tyrosine kinase-independent regulation of PLCγ in some systems. For example, in response to EGF, proline-rich regions of Akt interact with the SH3 domain of PLCγ1 resulting in association of the two enzymes, phosphorylation of PLCγ1 at Ser1248, and enhanced cellular motility. This finding demonstrates that PLCγ1 can function as a "scaffold" between RTKs and Akt, thereby establishing a mechanism by which the Akt signaling pathway cross-talks with tyrosine kinases. However, the mechanism and functional significance of phosphorylation at Ser1248 remains to be fully clarified, as it has also been shown that PKA-mediated phosphorylation at this site is inhibitory to PLCγ1 tyrosine phosphorylation and phospholipase activity in CD3-treated Jurkat cells suggesting that Ser1248 may be an allosteric regulator of PLCγ1 activity. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | D25A9 |
| Immunogen | Synthetic peptide corresponding to residues surrounding Ser1248 of human PLCγ1 protein. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser1248 of human PLCγ1 protein. |
| Usage | |
|---|---|
| Application | WB, IP, IF, IHC, FC |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:50) |
| MW(KDa) | 155 |
| Reactivity | Human, Mouse, Monkey |
| Sensitivity | Endogenous |
| Specificity | It recognizes endogenous levels of PLCγ1 protein only when phosphorylated at Ser1248. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.