Phospho-Numb (Ser276) Antibody - CD BioSciences

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Phospho-Numb (Ser276) Antibody

Phospho-Numb (Ser276) Antibody

SPA-08232

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Target Information
Target Name Numb
Gene Abbr. NUMB
Gene ID 8650
Full Name NUMB endocytic adaptor protein
Alias C14orf41, S171, c14_5527
Introduction Numb contains an amino-terminal phosphotyrosine-binding (PTB) domain and carboxy-terminal endocytic binding motifs for α-adaptin and EH (Eps15 homology) domain-containing proteins, indicating a role in endocytosis. There are four mammalian Numb splicing isoforms that are differentially expressed and may have distinct functions. Numb acts as a negative regulator of Notch signaling by promoting ubiquitination and degradation of Notch. The protein is asymmetrically segregated into one daughter cell during cell division, producing two daughter cells with different responses to Notch signaling and different cell fates. The localization of Numb can also be regulated by G-protein coupled receptor (GPCR) and PKC signaling.Numb can be phosphorylated at several sites including Ser7, Ser276 and Ser295. Phosphorylation at these sites regulates asymmetric membrane localization of Numb and integrin endocytosis.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Immunogen Synthetic phosphopeptide corresponding to Ser276 of the human Numb protein.
Source/Purification Antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to Ser276 of the human Numb protein. Antibodies are purified by protein A and peptide affinity chromatography.
Usage
Application WB
Dilutions Western Blot (1:1000)
MW(KDa) 78
Reactivity Human, Mouse, Rat, Monkey
Sensitivity Endogenous
Specificity It detects endogenous levels of Numb protein only when phosphorylated on Ser276.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol.
Preservative No Preservative
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.