Online Inquiry
Phospho-NF-κB p65 (Ser276) Antibody
SPA-07993
| Size | Price |
| 0.05 mL | Online Inquiry |
| 0.025 mL | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | NF-κB |
| Gene Abbr. | RELA |
| Gene ID | 5970 |
| Full Name | RELA proto-oncogene, NF-kB subunit |
| Alias | CMCU, NFKB3, p65 |
| Introduction | Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses. There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins. NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression. NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus.Methylation of NF-κB p65 by the methyltransferase SETD6 at Lys310 results in attenuated NF-κB signaling. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | Serum |
| Immunogen | RelA/NFkB p65 peptide corresponding to a region near [p Ser276] of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). |
| Usage | |
|---|---|
| Application | WB, ELISA, IHC |
| Dilutions | Western Blot (1:1000-1:5000); ELISA (1:10000-1:30000); Immunohistochemistry (1:200-1:800) |
| MW(KDa) | 65 |
| Reactivity | Human |
| Specificity | This phospho specific polyclonal antibody is specific for phosphorylated pS276 human p65. Reactivity with non-phosphorylated p65 is minimal. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Antiserum. |
| Preservative | 0.01% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.