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Phospho-NF-κB p65 (Ser276) Antibody
SPA-07991
| Size | Price |
| 0.05 mL | Online Inquiry |
| 0.025 mL | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | NF-κB |
| Gene Abbr. | RELA |
| Gene ID | 5970 |
| Full Name | RELA proto-oncogene, NF-kB subunit |
| Alias | CMCU, NFKB3, p65 |
| Introduction | Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses. There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins. NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression. NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus.Methylation of NF-κB p65 by the methyltransferase SETD6 at Lys310 results in attenuated NF-κB signaling. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | Synthesized phosphopeptide derived from human NF-kB p65 around the phosphorylation site of serine 276 (R-P-SP-D-R). |
| Usage | |
|---|---|
| Application | WB, IF, IHC |
| Dilutions | Western Blot (1:500-1:1000); Immunofluorescence (1:100-1:200); Immunohistochemistry (1:50-1:100) |
| MW(KDa) | 65 |
| Reactivity | Human, Mouse, Rat |
| Specificity | NkappaF-B p65 (specific to Phospho-Ser276) detects endogenous levels of NF-B p65 only when phosphorylated at serine 276. |
| Storage & Handling | |
|---|---|
| Storage Buffer | PBS (without Mg2+, Ca2+) (pH 7.4), 150 mM NaCl, 50% Glycerol. |
| Preservative | 0.02% Sodium Azide |
| Storage Temp. | Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.