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Phospho-Myosin Light Chain 2/MCL2 (Ser19) Antibody
SPA-07651
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | MLC2 |
| Gene Abbr. | MYL2 |
| Gene ID | 4633 |
| Full Name | myosin light chain 2 |
| Alias | CMH10, MLC-2s/v, MLC2 |
| Introduction | Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC), RLC, or LC20, has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner. This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction. ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers. Phosphorylation of smooth muscle MLC2 at Ser1/Ser2 and Ser9 by PKC and cdc2 has been reported to inhibit myosin ATPase activity. Phosphorylation by cdc2 controls the timing of cytokinesis. Transgenic mice lacking phosphorylation sites on the cardiac muscle isoform show morphological and functional abnormalities. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Ser19 of human myosin light chain 2. |
| Source/Purification | Antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser19 of human myosin light chain 2. Antibodies are purified by protein A and peptide affinity chromatography. |
| Usage | |
|---|---|
| Application | WB, IF |
| Dilutions | Western Blot (1:1000) |
| MW(KDa) | 18 |
| Reactivity | Human, Mouse, Rat, D. melanogaster |
| Sensitivity | Endogenous |
| Specificity | It detects endogenous levels of myosin light chain 2 (smooth muscle) only when phosphorylated at serine 19. The antibody does not cross-react with the cardiac isoform of myosin light chain 2. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol. |
| Preservative | No Preservative |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.