Online Inquiry
Phospho-mTOR (Ser2448) Antibody
SPA-07734
| Size | Price |
| 0.1 mg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | mTOR |
| Gene Abbr. | MTOR |
| Gene ID | 2475 |
| Full Name | mechanistic target of rapamycin kinase |
| Alias | FRAP, FRAP1, FRAP2, RAFT1, RAPT1 |
| Introduction | The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1. These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | This antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide corresponding a c-terminal region near Ser2448 of human TOR/mTOR. |
| Usage | |
|---|---|
| Application | WB, ELISA, IHC |
| Dilutions | Western Blot (1:500-1:2000); ELISA (1:10000-1:100000); Immunohistochemistry (5 µg/mL) |
| MW(KDa) | 289 |
| Reactivity | Human, Rat |
| Specificity | Reactivity occurs with phosphorylated mTOR from human derived tissues and cells. |
| Storage & Handling | |
|---|---|
| Storage Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2. |
| Preservative | 0.01% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.