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Phospho-MSK1 (Thr581) Antibody
SPA-08558
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | p90RSK |
| Gene Abbr. | RPS6KA5 |
| Gene ID | 9252 |
| Full Name | ribosomal protein S6 kinase A5 |
| Alias | MSK1, MSPK1, RLPK |
| Introduction | MSK1, a mitogen and stress activated protein kinase, is activated by Erk as well as p38 MAPK in response to growth factors and cellular stress, respectively. MSK1 resembles RSK because it has two kinase domains connected by a regulatory linker region. Phosphorylation of RSK1 at Ser364 and Ser381 is critical for RSK1 activity. These sites are analogous to Ser360 and Ser376 of MSK1, which may be important for MSK1 activity as well. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Thr581 of human MSK1. |
| Source/Purification | Antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr581 of human MSK1. Antibodies are purified by protein A and peptide affinity chromatography. |
| Usage | |
|---|---|
| Application | WB, IP, IHC |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:200) |
| MW(KDa) | 90 |
| Reactivity | Human, Mouse |
| Sensitivity | Endogenous |
| Specificity | It detects endogenous levels of MSK1 only when phosphorylated at Thr581. This antibody does not cross-react with MSK1 phosphorylated at other sites, nor does it detect phosphorylated RSK1, RSK2 or RSK3. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol. |
| Preservative | No Preservative |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.