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Phospho-MDM2/HDM2 (Ser185) Antibody
SPA-07325
| Size | Price |
| 0.1 mg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | MDM2 |
| Gene Abbr. | MDM2 |
| Gene ID | 4193 |
| Full Name | MDM2 proto-oncogene |
| Alias | ACTFS, HDMX, LSKB, hdm2 |
| Introduction | MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53. Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 177-198 of mouse MDM2. |
| Usage | |
|---|---|
| Application | WB, ELISA, IP |
| Dilutions | Western Blot (1:500-1:2000); ELISA (1:3000-1:12000); Immunoprecipitation (1:100) |
| MW(KDa) | 90 |
| Reactivity | Human |
| Specificity | Specific for phosphorylated MDM 2, giving a band in Western Blotting at approx 102 kD. |
| Storage & Handling | |
|---|---|
| Storage Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2. |
| Preservative | 0.01% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.