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Phospho-IRS-1 (Ser332/336) Antibody
SPA-06600
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | IRS-1 |
| Gene Abbr. | IRS1 |
| Gene ID | 3667 |
| Full Name | insulin receptor substrate 1 |
| Alias | HIRS-1 |
| Introduction | Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase. IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin. IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK and IKK while Ser789 is phosphorylated by SIK-2, a member of the AMPK family. The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively. Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity.GSK-3-mediated IRS-1 serine phosphorylation leads to inhibition of insulin-stimulated IRS-1 signaling. Ser332 and Ser336 of IRS-1 are situated in a glycogen synthase kinase-3 (GSK-3) consensus motif (SXXXS), and it has been shown that Ser332 is the actual GSK-3 phosphorylation site while Ser336 provides a " priming" site necessary for GSK-3 action. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Ser332/336 of mouse IRS-1 (equivalent to Ser337/341 of human IRS-1). |
| Source/Purification | Antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser332/336 of mouse IRS-1 (equivalent to Ser337/341 of human IRS-1). Antibodies are purified by peptide affinity chromatography. |
| Usage | |
|---|---|
| Application | WB |
| Dilutions | Western Blot (1:1000) |
| MW(KDa) | 180 |
| Reactivity | Rat |
| Sensitivity | Transfected Only |
| Specificity | It detects transfected levels of IRS-1 when phosphorylated at Ser332/336. It also detects IRS-1 protein when singly phosphorylated at Ser332 or Ser336 of human IRS-1. This antibody does not cross-react with other related phosphoproteins. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol. |
| Preservative | No Preservative |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.