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Phospho-Insulin Receptor β (Tyr1345) Antibody
SPA-06034
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | InsR |
| Gene Abbr. | INSR |
| Gene ID | 3643 |
| Full Name | insulin receptor |
| Alias | CD220, HHF5 |
| Introduction | Insulin receptor (InsR) is a heterodimeric membrane receptor tyrosine kinase. It is comprised of an extracellular α-subunit, containing the ligand binding domain, and a β-subunit containing an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. Binding of insulin to InsR results in receptor autophosphorylation, and subsequent tyrosine kinase activation. This provides a docking site for various adaptor molecules, including insulin-receptor substrate (IRS), Gab and Shc, phosphorylation of which promotes subsequent activation of multiple downstream signaling pathways including MAPK, PI3K and TC10. These events lead to increased glucose uptake and metabolism, and can promote cell growth. Loss of function mutation or desensitization of the InsR are two major contributors to insulin resistance and Type 2 diabetes. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | 14A4 |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Tyr1345 of human insulin receptor β. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1345 of human insulin receptor β. |
| Usage | |
|---|---|
| Application | WB |
| Dilutions | Western Blot (1:1000) |
| MW(KDa) | 95 |
| Reactivity | Human |
| Sensitivity | Transfected Only |
| Specificity | It detects transfected levels of insulin receptor β only when phosphorylated at Tyr1345. The antibody slightly cross-reacts with activated IGF-I receptors and some other activated tyrosine kinases. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.