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Phospho-IKKα (Ser180) Antibody
SPA-05468
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | IKKα |
| Gene Abbr. | CHUK |
| Gene ID | 1147 |
| Full Name | component of inhibitor of nuclear factor kappa B kinase complex |
| Alias | IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16 |
| Introduction | The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins. Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB. The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation. |
| Product Details | |
|---|---|
| Host | Rat |
| Clonality | Monoclonal |
| Clone No. | 829177 |
| Isotype | IgG2A |
| Immunogen | Phosphopeptide containing the human IKK alpha S176/S180. O15111. |
| Usage | |
|---|---|
| Application | WB |
| Dilutions | Western Blot (1 µg/mL) |
| MW(KDa) | 85 |
| Reactivity | Human |
| Specificity | Detects human IKK alpha when phosphorylated at S176/S180. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.