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Phospho-IKKα (Ser176)/IKKβ (Ser177) Antibody
SPA-05469
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | IKKα/β |
| Gene Abbr. | IKBKA/IKBKB |
| Gene ID | 1147 |
| Full Name | component of inhibitor of nuclear factor kappa B kinase complex |
| Alias | IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16 |
| Introduction | The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins. Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB. The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | C84E11 |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Ser177 of IKKβ. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser177 of IKKβ. |
| Usage | |
|---|---|
| Application | WB |
| Dilutions | Western Blot (1:1000) |
| MW(KDa) | 85 (alpha), 87 (beta) |
| Reactivity | Human, Mouse |
| Sensitivity | Endogenous |
| Specificity | It detects endogenous levels of IKKα and IKKβ only when phosphorylated at Ser176 and Ser177, respectively. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.