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Phospho-IκBα (Ser32/36) Antibody
SPA-08040
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | NF-κB Inhibitor |
| Gene Abbr. | NFKBIA |
| Gene ID | 4792 |
| Full Name | NFKB inhibitor alpha |
| Alias | EDAID2, IKBA, MAD-3, NFKBI |
| Introduction | The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins. Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB. IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | Synthesized phosphopeptide derived from human IkB-alpha. around the phosphorylation site of serine 32/36 (H-D-SP-G-L-D-SP -M-K). |
| Usage | |
|---|---|
| Application | WB, IF, IHC |
| Dilutions | Western Blot (1:500-1:1000); Immunofluorescence (1:100-1:200); Immunohistochemistry (1:50-1:100) |
| MW(KDa) | 40 |
| Reactivity | Human, Mouse |
| Specificity | IkB-alpha (specific to Phospho-Ser32/Ser36) detects endogenous levels of IkB-alpha only when phosphorylated at serine 32/36. |
| Storage & Handling | |
|---|---|
| Storage Buffer | PBS (without Mg2+, Ca2+) (pH 7.4), 150 mM NaCl, 50% Glycerol. |
| Preservative | 0.02% Sodium Azide |
| Storage Temp. | Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.