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Phospho-IκBβ (Ser23) Antibody
SPA-08034
| Size | Price |
| 0.025 mg | Online Inquiry |
| 0.05 mg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | NF-κB Inhibitor |
| Gene Abbr. | NFKBIB |
| Gene ID | 4793 |
| Full Name | NFKB inhibitor beta |
| Alias | IKBB, TRIP9 |
| Introduction | The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins. Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB. IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified.The regulation of IκBβ and IκBε is similar to that of IκBα. However, the phosphorylation and ubiquitin-mediated degradation of these proteins occurs with much slower kinetics. IKK phosphorylation of IκBβ occurs at Ser19 and Ser23, while IκBε can be phosphorylated at Ser18 and Ser22. The human sequence of IκB-β has also been reported to contain a threonine at position 19 suggesting that phosphorylation could be Thr19/Ser23. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | IkB-beta (Phospho-Ser23) A peptide sequence around phosphorylation site of serine 23 (L-G-S (p) -L-G) derived from Human IkappaB-b. |
| Usage | |
|---|---|
| Application | IHC |
| Dilutions | Immunohistochemistry (1:50-1:100) |
| MW(KDa) | 48-50 |
| Reactivity | Human, Mouse, Rat |
| Specificity | This antibody detects endogenous level of IkB-beta; only when phosphorylated at serine 23. |
| Storage & Handling | |
|---|---|
| Storage Buffer | PBS (without Mg2+, Ca2+) (pH 7.4), 150 mM NaCl, 50% Glycerol. |
| Preservative | 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Avoid freeze/thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.