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Phospho-HSL (Ser563) Antibody
SPA-05283
| Size | Price |
| 100 µL | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | HSL |
| Gene Abbr. | LIPE |
| Gene ID | 3991 |
| Full Name | lipase E, hormone sensitive type |
| Alias | AOMS4, FPLD6, HSL, LHS, REH |
| Introduction | HSL (hormone-sensitive lipase) catalyzes the hydrolysis of triacylglycerol, the rate-limiting step in lipolysis. Lipolytic stimuli activate adenylyl cyclase and thus increase intracellular cAMP levels, which in turn activate protein kinase A (PKA). PKA phosphorylates HSL at Ser563, Ser659, and Ser660, which stimulates HSL activity. In contrast, AMPK phosphorylates HSL at Ser565, which reduces HSL phosphorylation at Ser563 by PKA and inhibits HSL activity. Recent work indicates that phosphorylation at Ser600 by p44/42 MAPKs also enhances the enzymatic activity of HSL. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | A phospho specific peptide corresponding to residues surrounding S563 of human Hormone-sensitive Lipase/HSL. |
| Usage | |
|---|---|
| Application | WB |
| Dilutions | Western Blot (1:500-1:2000) |
| MW(KDa) | 81, 83 |
| Reactivity | Human |
| Storage & Handling | |
|---|---|
| Storage Buffer | PBS with 50% glycerol, pH 7.3. |
| Preservative | 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.