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Phospho-Histone H2Av (Ser137) Antibody
SPA-05103
| Size | Price |
| 0.1 mg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Histone H2A |
| Gene Abbr. | H2AC20 |
| Gene ID | 8338 |
| Full Name | H2A clustered histone 20 |
| Alias | H2A, H2A-GL101, H2A/q, H2AFQ, HIST2H2AC |
| Introduction | Variant histones H2A are synthesized throughout the cell cycle and are very different from classical S-phase regulated H2A. H2AvD is vital for viability, but the exact function of variant histones H2A is not known. H2A is a core component of the nucleosome, an octamer containing two molecules each of H2A, H2B, H3 and H4. The octamer wraps approximately 146 bp of DNA. HsAvD is expressed both maternally and zygotically and is found in embryos through to adults (female only). The human homologue, H2AX, is phosphorylated by ATM protein kinase when double strand DNA breaks occur. In mouse, H2AX "knock out" mice have an increased incidence of cancer. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | Histone H2Av [p Ser137]Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 132-141 of Drosophila melanogaster (fruit fly) H2Av protein. |
| Usage | |
|---|---|
| Application | WB, ELISA, IF, IHC |
| Dilutions | Western Blot (1:400-1:1600); ELISA (1:5000-1:20000); Immunofluorescence (1:10-1:500); Immunohistochemistry (2 µg/mL) |
| Reactivity | D. melanogaster |
| Specificity | This antibody is directed against the phosphorylated form of Drosophila Histone H2Av protein at the pS137 residue. |
| Storage & Handling | |
|---|---|
| Storage Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2. |
| Preservative | 0.01% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.