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Phospho-GRB10 (Ser476) Antibody
SPA-04815
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | GRB10 |
| Gene Abbr. | GRB10 |
| Gene ID | 2887 |
| Full Name | growth factor receptor bound protein 10 |
| Alias | GRB-IR, Grb-10, IRBP, MEG1, RSS |
| Introduction | The GRB7 family of adaptor proteins consist of GRB7, GRB10 and GRB14, which all contain an amino-terminal proline-rich SH3 binding domain, followed by PH, PBS, and SH2 domains. Each member of the family has several splice variants. It has been reported that GRB10 interacts with many receptor tyrosine kinases (RTKs) as well as downstream signal molecules including Raf, Akt, and Nedd4. Although it was originally thought that GRB10 is exclusively phosphorylated at serine residues Src kinase family members have been shown to phosphorylate GRB10 at Tyr67.Recently GRB10 was shown to be a direct substrate of mTORC1. It is phosphorylated by mTORC1 at Ser150, Ser428, and Ser476 upon insulin stimulation. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | D4E6 |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Ser476 of human GRB10 protein. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser476 of human GRB10 protein. |
| Usage | |
|---|---|
| Application | WB, IP |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:50) |
| MW(KDa) | 80 |
| Reactivity | Human, Mouse |
| Sensitivity | Endogenous |
| Specificity | This antibody recognizes endogenous levels of GRB10 protein only when phosphorylated at Ser476. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.