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Phospho-FLT3 (Tyr842) Antibody
SPA-04044
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | FLT3 |
| Gene Abbr. | FLT3 |
| Gene ID | 2322 |
| Full Name | fms related receptor tyrosine kinase 3 |
| Alias | CD135, FLK-2, FLK2, STK1 |
| Introduction | FMS-related tyrosine kinase 3 (FLT3, also called Flk2), is a member of the type III receptor tyrosine kinase family, which includes c-Kit, PDGFR and M-CSF receptors. FLT3 is expressed on early hematopoietic progenitor cells and supports growth and differentiation within the hematopoietic system. FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream targets. The p85 subunit of PI3 kinase, SHP2, GRB2 and Shc are associated with FLT3 after FL stimulation. Tyr589/591 is located in the juxtamembrane region of FLT3 and may play an important role in regulation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | 10A8 |
| Immunogen | Synthetic phosphopeptide corresponding to the sequences surrounding Tyr589/591 of human FLT3. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequences surrounding Tyr589/591 of human FLT3. |
| Usage | |
|---|---|
| Application | WB |
| Dilutions | Western Blot (1:1000) |
| MW(KDa) | 130 (nonglycosylated), 160 (glycosylated) |
| Reactivity | Human, Mouse |
| Sensitivity | Endogenous |
| Specificity | This antibody detects endogenous levels of FLT3 protein only when phosphorylated at tyrosine 589/591. The antibody does not cross-react with other phosphorylated tyrosine kinases. (US Patent No. 7,183,385 and foreign equivalents assigned to Cell Signaling Technology, Inc.) |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.