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Phospho-FLT3 (Tyr591) Antibody
SPA-04041
| Size | Price |
| 25 µg | Online Inquiry |
| 50 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | FLT3 |
| Gene Abbr. | FLT3 |
| Gene ID | 2322 |
| Full Name | fms related receptor tyrosine kinase 3 |
| Alias | CD135, FLK-2, FLK2, STK1 |
| Introduction | FMS-related tyrosine kinase 3 (FLT3, also called Flk2), is a member of the type III receptor tyrosine kinase family, which includes c-Kit, PDGFR and M-CSF receptors. FLT3 is expressed on early hematopoietic progenitor cells and supports growth and differentiation within the hematopoietic system. FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream targets. The p85 subunit of PI3 kinase, SHP2, GRB2 and Shc are associated with FLT3 after FL stimulation. Tyr589/591 is located in the juxtamembrane region of FLT3 and may play an important role in regulation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Tyr419 of human Src. |
| Source/Purification | Antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr419 of human Src. Antibodies are purified by protein A and peptide affinity chromatography. |
| Usage | |
|---|---|
| Application | WB |
| Dilutions | Western Blot (1:1000) |
| MW(KDa) | 130 (nonglycosylated), 160 (glycosylated) |
| Reactivity | Human, Mouse, Rat |
| Sensitivity | Endogenous |
| Specificity | This antibody detects endogenous levels of Src only when phosphorylated at tyrosine 416. The antibody may cross-react with other Src family members (Lyn, Fyn, Lck, Yes and Hck) when phosphorylated at equivalent sites. It does not cross-react with Src phosphorylated at tyrosine 527. It may cross react with phosphorylated RTKs. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol. |
| Preservative | No Preservative |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.