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Phospho-FLT3 (Tyr591) Antibody
SPA-04025
| Size | Price |
| 25 µg | Online Inquiry |
| 50 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | FLT3 |
| Gene Abbr. | FLT3 |
| Gene ID | 2322 |
| Full Name | fms related receptor tyrosine kinase 3 |
| Alias | CD135, FLK-2, FLK2, STK1 |
| Introduction | FMS-related tyrosine kinase 3 (FLT3, also called Flk2), is a member of the type III receptor tyrosine kinase family, which includes c-Kit, PDGFR and M-CSF receptors. FLT3 is expressed on early hematopoietic progenitor cells and supports growth and differentiation within the hematopoietic system. FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream targets. The p85 subunit of PI3 kinase, SHP2, GRB2 and Shc are associated with FLT3 after FL stimulation. Tyr589/591 is located in the juxtamembrane region of FLT3 and may play an important role in regulation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia.Tyr842 is located in the activation loop of FLT3. Mutation of Tyr842 was identified in human AML patients. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | Phosphopeptide containing human Flt-3 Y591 site. |
| Usage | |
|---|---|
| Application | WB, IF |
| Dilutions | Western Blot (0.5 µg/mL); Immunofluorescence (5-15 µg/mL) |
| MW(KDa) | 130 (nonglycosylated), 160 (glycosylated) |
| Reactivity | Human |
| Specificity | Detects human Flt-3 when phosphorylated at Y591. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.