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Phospho-eNOS (Ser1177) Antibody
SPA-03422
| Size | Price |
| 100 µL | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | eNOS |
| Gene Abbr. | NOS3 |
| Gene ID | 4846 |
| Full Name | nitric oxide synthase 3 |
| Alias | ECNOS, eNOS |
| Introduction | Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide a key regulator of blood pressure, vascular remodeling, and angiogenesis. The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495. Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli. In contrast, bradykinin and H2O2 activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | 1014F |
| Isotype | IgG |
| Immunogen | Phosphopeptide containing the human eNOS S1177 site. P29474. |
| Usage | |
|---|---|
| Application | WB |
| Dilutions | Western Blot (0.5 µg/mL) |
| MW(KDa) | 140 |
| Reactivity | Human |
| Specificity | Detects human eNOS when phosphorylated at S1177 in direct ELISAs and Western Blots. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | Sodium Azide |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.