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Phospho-eNOS (Ser116) Antibody
SPA-03420
| Size | Price |
| 0.1 mL | Online Inquiry |
| 0.025 mL | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | eNOS |
| Gene Abbr. | NOS3 |
| Gene ID | 4846 |
| Full Name | nitric oxide synthase 3 |
| Alias | ECNOS, eNOS |
| Introduction | Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide a key regulator of blood pressure, vascular remodeling, and angiogenesis. The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495. Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli. In contrast, bradykinin and H2O2 activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | Peptide corresponding to amino acids 106-122 of human eNOS/NOS III (Ser116). |
| Usage | |
|---|---|
| Application | WB |
| Dilutions | Western Blot (1:100-1:2000) |
| MW(KDa) | 140 |
| Reactivity | Human, Bovine |
| Specificity | eNOS/NOS III phosphorylated on serine 116. |
| Storage & Handling | |
|---|---|
| Storage Buffer | 0.2 M Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.1 mM EDTA and 30% Glycerol. |
| Preservative | 0.05% Sodium Azide |
| Storage Temp. | Aliquot and store at -20 °C or -80 °C. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.