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Phospho-cPLA2 (Ser505) Antibody
SPA-02324
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | cPLA2 |
| Gene Abbr. | PLA2G4A |
| Gene ID | 5321 |
| Full Name | phospholipase A2 group IVA |
| Alias | GURDP, PLA2G4, cPLA2, cPLA2-alpha |
| Introduction | Cytosolic phospholipase A2 (cPLA2) is a ubiquitously distributed enzyme that catalyzes the hydrolysis of the sn-2 acyl bond of glycerolipids to produce lysophospholipids and release arachidonic acid. cPLA2 has been implicated in diverse cellular responses such as mitogenesis, differentiation, inflammation and cytotoxicity. Calcium binding to the amino-terminal CalB domain of cPLA2 promotes the translocation of cPLA2 from cytosol to membrane, where cPLA2 cleaves arachidonic acid from natural membrane. Phosphorylation of cPLA2 by MAPK (p42/44 and p38) at Ser505 and Ser727 stimulates its catalytic activity. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | D4I2A |
| Immunogen | Synthetic peptide corresponding to residues surrounding Ser505 of human cPLA2 protein. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser505 of human cPLA2 protein. |
| Usage | |
|---|---|
| Application | WB, IP |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:100) |
| MW(KDa) | 95 |
| Reactivity | Human, Mouse |
| Sensitivity | Endogenous |
| Specificity | It recognizes endogenous levels of cPLA2 protein only when phosphorylated at Ser505. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.