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Phospho-Bid (Ser61) Antibody
SPA-01056
| Size | Price |
| 0.1 mL | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Bid |
| Gene Abbr. | BID |
| Gene ID | 637 |
| Full Name | BH3 interacting domain death agonist |
| Alias | FP497 |
| Introduction | Bid is a pro-apoptotic “BH3 domain-only” member of the Bcl-2 family originally discovered to interact with both the anti-apoptotic family member Bcl-2 and the pro-apoptotic protein Bax. Bid is normally localized in the cytosolic fraction of cells as an inactive precursor and is cleaved at Asp60 by caspase-8 during Fas signaling, leading to translocation of the carboxyl terminal p15 fragment (tBid) to the mitochondrial outer membrane. Translocation of Bid is associated with release of cytochrome c from the mitochondria, leading to complex formation with Apaf-1 and caspase-9 and resulting in caspase-9 activation. Thus, Bid relays an apoptotic signal from the cell surface to the mitochondria triggering caspase activation. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | The immunogen recognized by this antibody maps to phosphorylated Serine 61 and surrounding residues mouse BH3 interacting domain death agonist using the numbering given in entry NP_031570.2 (GeneID 12122). |
| Usage | |
|---|---|
| Application | WB |
| Dilutions | Western Blot (1:250-1:1000) |
| MW(KDa) | 15, 22 |
| Reactivity | Mouse |
| Storage & Handling | |
|---|---|
| Storage Buffer | Tris-Citrate/Phosphate (pH 7.0-8.0). |
| Preservative | 0.09% Sodium Azide |
| Storage Temp. | Store at 4 °C. |
| Handling | Do not freeze. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.