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Phospho-ATP-Citrate Lyase (Ser455) Antibody
SPA-00755
| Size | Price |
| 0.025 mg | Online Inquiry |
| 0.1 mg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | ATP-Citrate Lyase |
| Gene Abbr. | ACLY |
| Gene ID | 47 |
| Full Name | ATP citrate lyase |
| Alias | ACL, ATPCL, CLATP |
| Introduction | ATP-citrate lyase (ACL) is a homotetramer that catalyzes the formation of acetyl-CoA and oxaloacetate (OAA) in the cytosol, which is the key step for the biosynthesis of fatty acids, cholesterol and acetylcholine, as well as for glucogenesis. Nutrients and hormones regulate the expression level and phosphorylation of ATP-citrate lyase. It is phosphorylated by GSK-3 on Thr446 and Ser450. Ser455 of ATP-citrate lyase has been reported to be phosphorylated by PKA and Akt. Phosphorylation on Ser455 abolishes the homotropic allosteric regulation by citrate and enhances the catalytic activity of the enzyme. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Ser455 of human ATP-citrate lyase. |
| Source/Purification | Antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser455 of human ATP-citrate lyase. Antibodies are purified by protein A and peptide affinity chromatography. |
| Usage | |
|---|---|
| Application | WB, IP |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:50) |
| MW(KDa) | 125 |
| Reactivity | Human, Mouse |
| Sensitivity | Endogenous |
| Specificity | This antibody detects endogenous levels of ATP-citrate lyase only when phosphorylated at Ser455 (equivalent to Ser454 in rat). |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol. |
| Preservative | No Preservative |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.