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Phospho-Afadin (Ser1718) Antibody
SPA-00249
| Size | Price |
| 0.1 mg | Online Inquiry |
| 0.025 mg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Afadin |
| Gene Abbr. | AFDN |
| Gene ID | 4301 |
| Full Name | afadin, adherens junction formation factor |
| Alias | AF6, MLL-AF6, MLLT4, l-afadin |
| Introduction | In multicellular organisms, intercellular junctions play essential roles in tissue integrity and maintenance of cell polarity. Tight junctions (TJs) form a continuous barrier to fluids across the epithelium and endothelium. Adherens junctions (AJs) are dynamic structures that form cell-cell contacts linking cells into a continuous sheet. The actin filament-binding protein, Afadin, binds to nectin forming a connection to the actin cytoskeleton. AJs are formed when nectin assembles cadherin at the cell-cell adhesion site and these junctions are then involved in the formation and maintenance of TJs. Afadin has two splice variants: l-afadin, which is ubiquitously expressed, and s-afadin, which is expressed predominantly in neural tissue. s-Afadin is a shorter form lacking one of the three proline-rich regions found in l-afadin, as well as the carboxyl-terminal F-actin binding region. Human s-afadin is identical to AF-6, the ALL-1 fusion partner involved in acute myeloid leukemias. Recent work has also shown that afadin is involved in controlling the directionality of cell movement when it is localized at the leading edge of moving cells. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Ser1718 of human afadin. |
| Source/Purification | Antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1718 of human afadin. Antibodies are purified using protein A and peptide affinity chromatography. |
| Usage | |
|---|---|
| Application | WB |
| Dilutions | Western Blot (1:1000) |
| MW(KDa) | 205 |
| Reactivity | Human |
| Sensitivity | Endogenous |
| Specificity | This antibody detects endogenous levels of l-afadin protein only when phosphorylated at serine 1718. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol. |
| Preservative | No Preservative |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.