PHKG1 Knockout Cell Line - CD BioSciences

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PHKG1 Knockout Cell Line

PHKG1 Knockout Cell Line

SPL-02519

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1 Unit Online Inquiry
Description
2bp deletion
Target Information
Target Name PHKG1
Gene Abbr. PHKG1
Gene ID 5260
Full Name phosphorylase kinase catalytic subunit gamma 1
Alias PHKG
Species Human
Genomic Locus chr7:56087693
Transcript NM_006213
WT Expression Level 0.58 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing)
Introduction This gene is a member of the Ser/Thr protein kinase family and encodes a protein with one protein kinase domain and two calmodulin-binding domains. This protein is the catalytic member of a 16 subunit protein kinase complex which contains equimolar ratios of 4 subunit types. The complex is a crucial glycogenolytic regulatory enzyme. This gene has two pseudogenes at chromosome 7q11.21 and one at chromosome 11p11.12. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012].
Product Details
Cell Line Model HAP1
Genotype HAP1 cell line, edited by CRISPR/Cas to contain a 2bp deletion in a coding exon of PHKG1.
Description 2bp deletion
Parental Cell Line C631
Guide RNA Sequence GTCATCGACGTCACCGGTGG
PCR Primer Forward: TTGAGCCTGGGTGGTTCTAGTT
Reverse: AAGTGTAAATCTCTGTCAAGGGCAG
Handling Specifications
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish.
Culture Medium IMDM + 10% FCS
Growth Properties Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Freeze Medium IMDM + 20% FCS + 10% DMSO
Biosafety Level BSL-1
Disclaimer This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations.

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