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PERK Antibody
SPA-08784
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | PERK |
| Gene Abbr. | EIF2AK3 |
| Gene ID | 9451 |
| Full Name | eukaryotic translation initiation factor 2 alpha kinase 3 |
| Alias | PEK, PERK, WRS |
| Introduction | Protein kinase-like endoplasmic reticulum kinase (PERK) is an eIF2α kinase and transmembrane protein resident in the endoplasmic reticulum (ER) membrane that couples ER stress signals to translation inhibition. ER stress increases the activity of PERK, which then phosphorylates eIF2α to promote reduced translation. Research studies have demonstrated that PERK-deficient mice have defects in pancreatic β cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress. PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain. Phosphorylation of PERK at Thr980 serves as a marker for its activation status. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | Serum |
| Immunogen | This whole rabbit serum was prepared by repeated immunizations with a recombinant fusion protein from amino acids 601-1115 of mouse PERK (see link below for the full length sequence of the mouse gene product). |
| Usage | |
|---|---|
| Application | WB, ELISA, IF, IHC, IP |
| Dilutions | Western Blot (1:500-1:3000); ELISA (1:4000-1:20000); Immunofluorescence (1:10-1:500); Immunohistochemistry (1:10-1:500); Immunoprecipitation (10-30 µL) |
| MW(KDa) | 140 |
| Reactivity | Mouse |
| Validation | Knockout Validated. |
| Storage & Handling | |
|---|---|
| Storage Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2. |
| Preservative | 0.01% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.