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PDGFRA (D842V/+) Cell Line
SPL-02495
| Size | Price |
| 1 Unit | Online Inquiry |
| Description |
|---|
| PDGFRA (D842V/+) |
| Target Information | |
|---|---|
| Target Name | PDGF Receptor |
| Gene Abbr. | PDGFRA |
| Gene ID | 5156 |
| Full Name | platelet derived growth factor receptor alpha |
| Alias | CD140A, PDGFR-2, PDGFR2 |
| Species | Human |
| Genomic Locus | 4q12 |
| Introduction | This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers. [provided by RefSeq, Mar 2012]. |
| Product Details | |
|---|---|
| Cell Line Model | HCT116 |
| Genotype | PDGFRA (D842V/+) |
| Description | PDGFRA (D842V/+) |
| Parental ATCC ID | CCL-247 |
| STR Profile | Amelogenin: X,Y; CSF1PO: 7,10; D13S317: 10,12; D16S539: 11,13; D5S818: 10,11; D7S820: 11,12; THO1: 8,9; TPOX: 8,9; vWA: 17,22; ATCC |
| Disease | Colorectal carcinoma |
| Tissue | Colon |
| Gender | Male |
| Key Endogenous Mutations | CDKN2A, CTNNB1, KRAS, PIK3CA, MLH1 |
| CVT Selection Condition | G418 resistant - 0.3 mg/mL |
| Cell Number | 1x10^6 |
| Handling Specifications | |
|---|---|
| Revival | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 70-80% confluency, approximately 1:10-1:20. |
| Culture Medium | RPMI 1640 including 2 mM L-glutamine and 25 mM sodium bicarbonate, supplemented with 10% FBS. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 70-80% confluency, approximately 1:10-1:20. |
| Freeze Medium | Complete growth media 45% + USDA approved FBS 50% + DMSO 5% |
| Biosafety Level | BSL-1 |
| Disclaimer | This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.