PARP2 Knockout Cell Line - CD BioSciences

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PARP2 Knockout Cell Line

PARP2 Knockout Cell Line

SPL-02453

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Description
2bp deletion
Target Information
Target Name PARP2
Gene Abbr. PARP2
Gene ID 10038
Full Name poly(ADP-ribose) polymerase 2
Alias ADPRT2, ADPRTL2, ADPRTL3, ARTD2, PARP-2
Species Human
Genomic Locus chr14:20343651
Transcript NM_005484
WT Expression Level 70.70 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing)
Introduction This gene encodes poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jul 2008].
Product Details
Cell Line Model HAP1
Genotype HAP1 cell line, edited by CRISPR/Cas to contain a 2bp deletion in a coding exon of PARP2.
Description 2bp deletion
Parental Cell Line C631
Guide RNA Sequence CGGCGGCGACGGAGCACCGG
PCR Primer Forward: CAGAACACATAGCGACATGCAAATA
Reverse: ATGTAATCCCCAAAGCTCTGAAAAC
Handling Specifications
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish.
Culture Medium IMDM + 10% FCS
Growth Properties Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Freeze Medium IMDM + 20% FCS + 10% DMSO
Biosafety Level BSL-1
Disclaimer This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations.

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