Online Inquiry
p58IPK Antibody
SPA-08464
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | p58IPK |
| Gene Abbr. | DNAJC3 |
| Gene ID | 5611 |
| Full Name | DnaJ heat shock protein family (Hsp40) member C3 |
| Alias | ACPHD, ERdj6, HP58, P58, P58IPK |
| Introduction | p58IPK is an inhibitor of interferon-induced and double-stranded RNA-activated protein kinase (PKR). It physically interacts with PKR and inhibits its activation and activity. Influenza virus activates p58IPK and thus blocks the activity of PKR to repress translation in the infected cells. In the uninfected cells, p58IPK forms a complex with its own inhibitor, HSP40, and is kept in an inactive state. ER stress induces the expression of p58IPK mediated by ATF6. The induced p58IPK negatively regulates Perk activity, inhibits eIF2α phosphorylation and suppresses the activation of expression of downstream ER-responsive genes ATF4 and GADD153. More recently, p58IPK has been shown to associate with the lumen of the endoplasmic reticulum (ER) where it is believed to serve as a cochaperone for BiP. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | C56E7 |
| Immunogen | Synthetic peptide corresponding to the sequence of human p58IPK. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human p58IPK. |
| Usage | |
|---|---|
| Application | WB, IP |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:50) |
| MW(KDa) | 58 |
| Reactivity | Human, Mouse, Monkey |
| Sensitivity | Endogenous |
| Specificity | Detects endogenous levels of total p58IPK protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.