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p130 Cas Antibody
SPA-08310
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | p130 Cas |
| Gene Abbr. | BCAR1 |
| Gene ID | 9564 |
| Full Name | BCAR1 scaffold protein, Cas family member |
| Alias | CAS, CAS1, CASS1, CRKAS, P130Cas |
| Introduction | p130 Cas (Crk-associated substrate) is a docking protein containing multiple protein-protein interaction domains. The amino-terminal SH3 domain may function as a molecular switch regulating CAS tyrosine phosphorylation, as it interacts with focal adhesion kinase (FAK) and the FAK-related kinase PYK2 as well as the tyrosine phosphatases PTP-1B and PTP-PEST. The carboxy-terminal Src binding domain (SBD) contains a proline-rich motif that mediates interaction with the SH3 domains of Src-family kinases (SFKs) and a tyrosine phosphorylation site (Tyr668 and/or Tyr670) that can promote interaction with the SH2 domain of SFKs. The p130 Cas central substrate domain, the major region of tyrosine phosphorylation, is characterized by 15 tyrosines present in Tyr-X-X-Pro (YXXP) motifs, including Tyr165, 249, and 410. When phosphorylated, most YXXP motifs are able to serve as docking sites for proteins with SH2 or PTB domains including adaptors, C-Crk, Nck, and inositol 5'-phosphatase 2 (SHIP2). The tyrosine phosphorylation of p130 Cas has been implicated as a key signaling step in integrin control of normal cellular behaviors including motility, proliferation, and survival. Aberrant Cas tyrosine phosphorylation may contribute to cell transformation by certain oncoproteins. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | E1L9H |
| Immunogen | Synthetic peptide corresponding to residues surrounding Leu116 in the SH3 domain of human p130 Cas protein. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu116 in the SH3 domain of human p130 Cas protein. |
| Usage | |
|---|---|
| Application | WB, IP, IF, IHC, FC |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:100) |
| MW(KDa) | 130 |
| Reactivity | Human, Mouse, Rat, Monkey |
| Sensitivity | Endogenous |
| Specificity | It recognizes endogenous levels of total p130 Cas protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.