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NRAS (Q61K/+/+) Cell Line
SPL-02334
| Size | Price |
| 1 Unit | Online Inquiry |
| Description |
|---|
| NRAS (Q61K/+/+) |
| Target Information | |
|---|---|
| Target Name | NRAS |
| Gene Abbr. | NRAS |
| Gene ID | 4893 |
| Full Name | NRAS proto-oncogene, GTPase |
| Alias | ALPS4, CMNS, N-ras, NCMS, NRAS1 |
| Species | Human |
| Genomic Locus | 1p13.2 |
| Introduction | This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]. |
| Product Details | |
|---|---|
| Cell Line Model | A375 |
| Genotype | NRAS (Q61K/+/+) |
| Description | NRAS (Q61K/+/+) |
| Parental ATCC ID | CRL-1619 |
| STR Profile | Amelogenin: X; CSF1PO: 11,12; D13S317: 11,14; D16S539: 9; D5S818: 12; D7S820: 9; THO1: 8; TPOX: 8,10; vWA: 16,17 |
| Disease | Malignant melanoma |
| Tissue | Skin |
| Gender | Female |
| Key Endogenous Mutations | BRAF, CDKN2A |
| CVT Selection Condition | G418 resistant - 0.65 µg/mL |
| Cell Number | 1x10^6 |
| Handling Specifications | |
|---|---|
| Revival | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 70-80% confluency, approximately 1:5-1:6. |
| Culture Medium | DMEM/F-12 including 2.5 mM L-glutamine and 15 mM HEPES, supplemented with 10% FBS. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 70-80% confluency, approximately 1:5-1:6. |
| Freeze Medium | Complete growth media 40% + USDA approved FBS 50% + DMSO 10% |
| Biosafety Level | BSL-1 |
| Disclaimer | This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.