NOTCH4 Sandwich ELISA Kit - CD BioSciences

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NOTCH4 Sandwich ELISA Kit

NOTCH4 Sandwich ELISA Kit

SPK-00875

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Target Information
Target Name Notch4
Gene Abbr. NOTCH4
Gene ID 4855
Full Name notch receptor 4
Alias INT3
Introduction Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate. Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer comprised of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes.Notch4 is primarily expressed in endothelial cells and is processed similarly to Notch1. The mouse Notch4 gene (also known as Int-3) is a frequent target in Mouse Mammary Tumour Virus (MMTV)-induced mammary tumors. The intracellular domain of Notch4 binds to Smad3 and inhibits TGF-β signaling.
Product Details
Measurement Sandwich
Usage
Application ELISA
Reactivity
Assay Principle quantitative
Sensitivity 19.5 pg/mL
Detection Range 78 pg/mL - 5000 pg/mL
Sample Volume 50-100μL
Assay Time 1-5h
Detection Wavelength 450 nm
Precision Intra-assay Precision (Precision within an assay): CV%<8%.
Inter-assay Precision (Precision between assays): CV%<10%.
Storage & Handling
Storage Temp. Store at 2-8°C. Please refer to protocol.

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