NOS2 Knockout Cell Line - CD BioSciences

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NOS2 Knockout Cell Line

NOS2 Knockout Cell Line

SPL-02311

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1 Unit Online Inquiry
Description
2bp deletion
Target Information
Target Name iNOS
Gene Abbr. NOS2
Gene ID 4843
Full Name nitric oxide synthase 2
Alias HEP-NOS, INOS, NOS, NOS2A
Species Human
Genomic Locus chr17:27788861
Transcript NM_000625
WT Expression Level 0.42 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing)
Introduction Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008].
Product Details
Cell Line Model HAP1
Genotype HAP1 cell line, edited by CRISPR/Cas to contain a 2bp deletion in a coding exon of NOS2.
Description 2bp deletion
Parental Cell Line C631
Guide RNA Sequence TTTTTGATCCTCACATGCCG
PCR Primer Forward: TAACAGGGAGTTGGCATCATTTACT
Reverse: ATAGATCCTTCCCCACTCTAAGACA
Handling Specifications
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish.
Culture Medium IMDM + 10% FCS
Growth Properties Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Freeze Medium IMDM + 20% FCS + 10% DMSO
Biosafety Level BSL-1
Disclaimer This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations.

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