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NF-κB2 p100/p52 Antibody
SPA-07959
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | NF-κB |
| Gene Abbr. | NFKB2 |
| Gene ID | 4791 |
| Full Name | nuclear factor kappa B subunit 2 |
| Alias | CVID10, H2TF1, LYT-10, LYT10, NF-kB2 |
| Introduction | Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses. There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins. NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression. NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus. |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | 291319 |
| Isotype | IgG1 |
| Immunogen | E. coli-derived recombinant human NF kappa B2. Met1-Asn447. Q00653. |
| Usage | |
|---|---|
| Application | WB, ChIP, IF |
| Dilutions | Western Blot (0.5 µg/mL); Chromatin Immunoprecipitation (5 µg/5x10^6 cells); Immunofluorescence (3-25 µg/mL) |
| MW(KDa) | 52 (mature), 120 (precursor) |
| Reactivity | Human |
| Specificity | Detects human NFkB2 in direct ELISAs and Western Blots. In Western Blots, no cross-reactivity with endogenous mouse NFkB2 in NIH 3T3 cells is observed. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.